Since last week's electrophoresis gel was not very successful, we re-did the procedure and hoped for better results. My mentor crated the solutions again that way I had time to re-imbed the gel with our DNA, and take it down to examine under the UV light.
Thankfully, our gel worked this time and we were able to see the DNA bands under the UV light. We cut out the bands of DNA and saved them in test tubes, and later I will use them in gel extraction and sequencing further down my project.
Then after that we went to work on isoform 3. Since these cells were already ready to be put into the cell all we had to do was add lipids to the membrane of the DNA to ensure that the plasmid would be engulfed ny the cell. If the DNA did not have a lipid membran the cell would not take in the DNA that we want it to. With the plasmid we are also going to add an antibiotic resistance and then when we need to we can add antibiotic to our cells and the ones that live are the ones that obsorbed the plasmid with DNA. and those are the cells that will benefit us in our experiment.
